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To confirm the effect of phloroglucinol, the invasiveness of cancer cells were also analyzed in the presence or absence of phloroglucinol by 3D-culture system, where common extracellular matrix components such as collagen I and matrigel were mixed and solidified in growth medium, mimicking in vivo tissue environment.
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Ten μL of cell suspension containing SW620 cells (3 × 10), 20 μL of 2× IMDM and 30 μL 0.8% Bacto agar (55°C) was mixed and transferred to the solidified pre-layer in each well.
He has really energized and solidified thousands of people who wouldn't have gone to the polls otherwise".
They have harmed ordinary people and solidified sentiment against the west and the "great Satan" of the US.
Through the nineties, Norcross extended his political operation beyond Camden and solidified control over three other southern counties and several municipalities by recruiting and financing his own candidates.
I've been known to have a Boxing Day sandwich composed of turkey, cranberry, stuffing, jellified gravy and solidified bread sauce (recommended).
Some rooms have been partially preserved by the 15m (49ft) of ash and solidified lava that covered the house.
The slag is formed by small drops of molten and solidified steel in contact with the substrate material.
500 µl of microbial cultures of age 18 24 h were inoculated into Petri plates in nutrient agar medium and solidified.
As Falah entered her teens, Lark's presence grew and solidified.
This prevarication brought a gasp from the assembled faculty and solidified faculty opposition to the Summers presidency.
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