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Finally, the mold contaminated diet was naturally air-dried, mixed, and sampled for detection of mycotoxins.
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To 10 μg of protein, 10 μl of lamellae buffer (loading dye) was mixed and samples were incubated at 95 °C for 5 min in water bath and then immediately load the protein sample into the wells.
Supernatant and agarose pellets were mixed, and samples were loaded on sodium dodecyl sulfate-polyacrylamide gel electrophoresis gel (Invitrogen) and blotted to a nitrocellulose membrane.
Thirty µl of 10 mM hydroquinone (Sigma) and 520 µl of 3 M sodium bisulfite (Sigma) at pH 5.0 both freshly prepared, were added and mixed, and samples were incubated at 50°C for 16 h.
The quality of studies was mixed, and sample sizes were generally small.
The composite soil samples were well mixed and sub-samples taken.
To each drag swab sample, 100 mL of BPW were added, the bag was mixed, and the sample was incubated at 42°C overnight.
Urine was collected for 24 h, uniformly mixed, sampled, and then stored at −80 °C until subsequent analyses.
Lineages 6 and 7 appear in both mixed and unmixed samples.
The RNA extracts labeled with Alexa 488 was collected from all tissue samples, mixed and used as reference sample referred to here as a the common reference.
The array could be used to detect individual Fusarium species in mixed samples and in environmental samples.
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