Sentence examples for mixed and fractionated from inspiring English sources

The four labelled samples were then mixed and fractionated into 12 fractions by strong cation exchange (SCX) HPLC.

An equal ratio (1 1) of treated and untreated MDA-MB-231 cells were mixed and fractionated using the ProteoJET™ Cytoplasmic and Nuclear Protein Extraction Kit K0311 (Fermentas, Canada) according to the manufacturer's instructions.

The basic principle of 2D-DiGE is that protein extracts from two different biological situations (in this case HeLa cells treated with a control or targeted siRNA) are differentially labelled with fluorescent dyes before being mixed and fractionated by 2D gel electrophoresis [ 43, 44].

Peptides derived from a corresponding set of samples, comprising eight experimental traits per age group, were labeled using the iTRAQ 8plex kit (ABsciex, Framingham, MA, USA), as shown in Table S 1. Peptides were then mixed and fractionated simultaneously, using isoelectric focusing (IEF) on 24 cm IPG strips (pH 3.5 to 4.5; GE Healthcare), as described previously (Mikula et al. 2011).

The labeled peptides from both sets were mixed and fractionated using HILIC chromatography (hydrophilic interaction liquid chromatography).

Following digestion, we labeled the samples with the TMT reagents, mixed the labeled peptides, and fractionated the mixtures using offline basic-pH reverse phase HPLC.

The digested peptides were desalted, dried under vacuum, reconstituted in 10% acetonitrile, and fractionated using mixed mode ion chromatography with a Polycat A column and Polywax LP column in series (PolyLC Inc., Columbia, MD).

Air dried fruits were extracted with ethanol and fractionated into four fractions.

After labeling, samples were pooled, desalted and concentrated using a mixed mode cation exchange (MCX) cartridge (Waters), and fractionated by preparative isoelectric focusing using a Free Flow Electrophoresis (FFE, BD Biosciences, Inc).

Protein samples were run under reducing conditions 20 μg of protein lysate was mixed with Laemmli buffer (Bio-Rad #161 0737), containing 5% 2-mercaptoethanol (Sigma M6250), and fractionated by SDS-PAGE using the Mini-PROTEAN® Tetra System and pre-cast TGX™ Gels (Bio-Rad #456-1096); Spectra™ Multicolor Broad Range Protein Ladder Thermo #26634) was used as a reference.

One μl of the product was mixed with Hi-Di formamide (Applied Biosystems Inc., Foster City, US) and Internal Lane Standard (ILS600), denatured and fractionated on an ABI 3730 Genetic Analyzer (Applied Biosystems Inc).