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"I had to have the whole orchestra mixed and amplified to me.
Aliquots of both PCR products were then mixed and amplified using the previously used 5' primer for the OmpA fragment and the 3' primer for boophilin.
(2) The primers PD_up, PD_down, and phage Lambda (Table 1) were mixed and amplified as follows: 2 min at 94°C, followed by 25 cycles of 30 s at 94°C, 30 s at 30°C, and 30 s at 72°C.
For this purpose, known proportions of normal DNA (derived from leukocytes) and tumor DNA were mixed and amplified [ 15].
For this study we mixed and amplified known quantities and proportions of normal leukocyte and serum DNA, as described before [ 18].
DNA of positive clones were mixed and amplified for 30 cycles under the following conditions: denaturation for 30 s at 94°C, annealing for 45 s at 49°C, and extension for 50 s at 72°C and double restriction digestion, followed by agarose gel analysis.
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Next, 2 µg of cDNA was mixed with 0.9 µM TaqMan primers and master mix and amplified at 60°C in a Mx3005P (Stratagene qPCR system).
The cDNA was mixed with 0.9 μM TaqMan primers and master mix and amplified at 60°C in a Mx3005P thermocycler (Stratagene).
cDNA was mixed with 2x Taqman PCR mix (AB) and amplified customized TLDA (TaqMan Low Density Array) using Abiprism 7900HT apparatus (AB).
More specifically, we amplified the inserts of the cDNA clones and mixed the amplified products to obtain their compound SSCP and HD banding patterns, and then compared these patterns with those obtained from PCR products amplified from the original cDNA.
The two PCR products were mixed, allowed to anneal, and amplified using two primers, one (1915) complementary to the 5' end of the aph gene and a second (1918) complementary to the 3' end of xylE to produce a 1785-bp fragment.
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