Sentence examples for mix which consisted from inspiring English sources

Sodium bisulfite-converted DNA (15 ng) was added to the PCR reaction mix, which consisted of high-resolution melting Master Mix (Roche Applied Science), 0.25 μM primers, and Mg2+ (2.5 mM).

For the CE-SSCP analyses, the fluorescent-labelled PCR samples were prepared for electrophoresis by combining 1 μl PCR product with 14 μl loading mix which consisted of 13.75 μl Hi-DI formamide and 0.25 μl Genescan ROX 350 standard (Applied Biosystem).

Briefly, three uL of total RNA (350-1000ng) waddedded to 4.50uL of RT reaction mix, which consisted of 10x Megaplex RT Primers, 100mM dNTPs with dTTP, 50U/uL MultiScribe Reverse Transcriptase, 10x RT buffer, 25mM MgCl, 20U/uL RNase Inhibitor, and nuclease-free H2O.

2×10 FLK1+ cells collected from day 3.5 3 EBs were seeded in methylcellulose mix which consisted of IMDM supplemented with 55% methylcellulose (BioScientific), 10% FBS pretested for efficient EB differentiation, 1% L-glutamine, 1% L-glutamine, 0.6% 30 mg/ml transferrin, 0.3% 0.15 M MTG, 0.5% 5 mg/ml ascorbic acid, 15% D4T conditioned media, 0.1% 5 µg/ml VEGF, and 0.1%10µg/mlml IL-6.

This study adopted the mixed-methods sequential explanatory design which consisted of two consecutive phases, first quantitative, then qualitative, in data collection and analysis.

For the Na+./K+-ATPase activity measurement, the isolated stria vascularis capillary homogenates were mixed in the reaction buffer which consisted of 50 mmol/L Tris, 2.5 mmol/L MgCl2 and 0.5 mmol/L ATP.

Figures4 and5 compare the results above with mixing fuel which consists of coal and biomass.

That package, which consisted of a mix of spending and tax cuts, totalled about two per cent of G.D.P. each year.

Target gene expression levels were normalized to the endogenous reference values, and then normalized to a calibrator sample, which consisted of a mix of cDNA from each timepoint.

According to the manufacturer's instruction, 5 μl nucleic acid was added to the Master Mix, which consisted of 18 μl Super Mix, 1 μl Enzyme Mix and 1 μl Internal Control.

They served a beverage they called Mother Hunch Punch, which consisted of equal parts fruit juice and clear liquor, and was mixed in a large barrel in the back yard.