Sentence examples for mix were performed from inspiring English sources

Revertant colonies seen with 20  μM B[a]P with S9 mix were performed as a positive control.

Shorter polishing steps, alternated with etching steps of 5 seconds in a 75% (v/v) acetic acid (glacial) and 25% hydrogen peroxide (100 volumes) mix, were performed in order to remove attached abrasive particles.All the samples were stored in a desiccator for 24 hours before the beginning of the test.

Each mix was performed in duplicate.

First strand cDNA synthesis and quantitative real-time PCR with SYBR green master mix was performed in Stratagene MX4000 Real Time PCR System®.

When these gave conflicting results, another replicate mix was performed and the majority result was counted.

Transformation of the ligation mix was performed using the DH5αMAX competent cells (Invitrogen, Carlsbad, CA, USA).

Real-time PCR using TaqMan Universal PCR master mix was performed to analyze RNA expression levels with Applied Biosystems Standard Real-Time PCR systems.

If both clones were in two different sets, then that particular mix was performed 4 times (for example, QSvi9 and QSvi29).

Two-step RT-PCR using the SYBR® Green PCR Master Mix was performed according to the manufacturer's protocol and universal thermal cycling parameters (Applied Biosystems).

After the ethanol-washing step (using 1 ml of 100% ethanol), pellets were air-dried and digestion with Proteinase K in 0.25 ml digestion mix was performed at 55°C for 15 min.

First-strand cDNA synthesis and quantitative real-time PCR with SYBR green master mix was performed in an Opticon 2 DNA engine (BioRad Corp., Hercules, CA, USA) according to the manufacturer's instructions.