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Control samples (MIX) were generated from randomly laser microdissected cells from the ventral part of the L2 spinal cord sections.
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While the company is selling only wind-generated power in Texas, Kelly concedes that the biggest component of its power mix is generated by natural gas and hydroelectric power.
Labelled cRNA was fragmented and a hybridisation mix was generated as recommended (Affymetrix, 1998).
In order to simulate a more "realistic" environmental condition, "EPA DEP MIX" was generated by mixing DEPs collected at three different engine operations.
Briefly, a 2× target mix was generated containing 125 ng of cyanine 3-labeled cRNA, 125 ng of cyanine 5-labeled cRNA, appropriate amounts of labeled synthetic target, and 25 μL of Agilent's 10× control solution in a final volume of 125 μL.
Assay mixes were generated for both amplification of cortex and the reference gene rpS4.
We find that 98% of the power expended in mechanical stirring produces no detectable mixing at all, and in fact convective mixing is generated solely by tiny perturbations to a dominant non-chaotic flow.
The starting pool of mutants (mutIV-mix) was generated through four rounds of mutagenesis (2 X UV at 50 J/m and 2 X MMS at 0.1v%/1v% for 1 min).
Mixed multimer or trans-complemented vectors were generated by mixing equal amounts of the fusion protein-containing and either wild-type (wt) IN or inactive IND64V-coding packaging plasmids.
Mixed bone marrow chimeras were generated by intravenously transferring 3 × 10 to 5 × 10 cells from the following mixes into lethally irradiated (2 × 450 rads, 3 hr apart) CD45.1+ congenic mice: blt/ blt CD45.2+: WT CD45.1+CD45.2+; blt/+ CD45.2+: WT CD45.1+CD45.2+, at a 50 50 ratio.
Volatile species were generated mixing acidified aqueous samples and sodium tetrahydroborate solution in a continuous flow system, and the chemical and instrumental conditions were optimised experimentally.
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