Sentence examples for mix was washed from inspiring English sources

The mix was washed three times with lysis buffer.

The resin slurry and supernatant mix was washed with increasing imidazole concentrations, respectively, 10 mM and 20 mM.

Cells were transfected using jetPRIME (Polyplus transfection, 114-01), and the transfection mix was washed off after 4 h, and assayed 24 h later.

The cohesin-DNA bead mix was washed with high salt (500 mM KCl) to remove any free cohesin or cohesin not stably bound to DNA.

The hybridization mix was washed with wash buffers of different stringency in the presence of magnetic beads (Streptavidin Dynabeads, Life Technologies) at 47°C and the eluate was post-capture PCR amplified (18 cycles) with Illumina PCR primers appropriate to the kit used.

A mixed-stage worm culture was washed of the plates with M9 buffer, eggs were prepared by hypochlorite treatment and were allowed to hatch over night in M9 buffer.

After an overnight incubation in the transfection mix, cells were washed and fed with EGM-2MV.

FDCP-mix cells were washed and resuspended (4 × 10 cells ml−1) in Fisher's medium supplemented with 20% (v v−1) HS.

After 30 minutes of mixing, the beads were washed four times.

Then 500 μg of each sample was incubated with HA beads overnight at 4°C with mixing, then beads were washed five times with RIPA buffer.

After incubating the supernatants with the beads for 1 hour at 4°C with end-over-end mixing, the beads were washed five times with 1 mL of lysis buffer and the immunoprecipitates eluted with 15 µL of SDS sample buffer.