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The DNAfectin mix was then added to the plasmid mix and gently mixed and incubated for 20 min (about 60 μl total for each well).
The FuGENE® DNA mix was then added to 10 ml Schneider's medium and mixed thoroughly.
The optimized mix was then employed for AAM FC production by using an H2O2 blowing agent (gas-foaming method).
The molten mix was then rapidly poured onto a plate of the same medium with 1% agar, and allowed to set.
The heterocoagulated mix was then spray-dried in order to avoid any separated coagulation of pigment and matrix and to obtain a well granulated powder suitable for application in ceramic bodies.
The mix was then sonicated for 2 additional hours at 65 °C.
The acetonitrile:water mix was then decreased to 50 50 (1.0 mL/min) during the next 10 min.
Two and a half microliters of the PI mix was then added to the mixture, and the resulting cell suspension was held at 4°C in the absence of light for 15 min.
A 24-track mobile studio piped Gilmour's Fender tracks through a public address system, and the resulting mix was then recorded in surround sound.
One µl of each 20-µl reaction mix was then used for amplification by PCR.
The reaction mix was then added to 100 µl chemically competent DH10b cells, incubated for 15 30 min on ice and transformed by heat shock.
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