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The reaction mix was supplemented with 1 µl EDTA (25 mM), followed by incubation for 10 min at 22°C and transformation of the whole reaction mix into E. coli for plasmid amplification.
The mix was supplemented with 3 µl each of 100 mM DTT and 10 mM ATP, 1 µl ligase, 0.5 µl each of BamHI and BglII, and was incubated overnight at room temperature.
The mix was supplemented with 2.5 mM ATP and oxygen scavenging system.
When PCR mix was supplemented with SGI and isolated DNA was amplified, a different picture was observed.
The RT-PCR mix was supplemented with 0.2X Titanium Taq polymerase (BD Biosciences, Palo Alto, CA) to improve primer efficiency.
The potting mix was supplemented with 8 g of Osmocote ® (The Scott's Miracle Gro Company, Marysville, OH) per pot at the time of planting.
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Excellent performance was found when PCR mix II was supplemented with PT enhancer.
Interestingly, strong and specific amplification was observed when PCR mix II was supplemented with both 1 M 1,2-propanediol and 0.2 M trehalose.
When PCR mix II was supplemented with SYTO-9, SYTO-13, EvaGreen, LCGreen or ResoLight, no dramatic changes in mutation frequencies were observed (Table 3).
The SYBR green PCR master mix (Applied Biosystems) was supplemented with 1 ρmol of each gene-specific oligonucleotide and 40 ng of template cDNA in a final volume of 20 μl.
The binders' mix-design was supplemented with sorptive clays to prevent migration of toxic elements from fly ash.
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