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Five mL MEBS [malt extract broth with 10.8% (w/v) sucrose] containing 150 μl transformation mix was distributed over five MEAS-plates [malt-extract agar with 10.8% (w/v) sucrose].
When multiple plasmids were tested in the same experiment, the master mix was distributed among the treatment wells before the addition of DNA.
The transfection mix was distributed into the siRNA library plates.
Master Mix was distributed to 0.2-mL PCR tubes, and 5 µL of template cDNA was added.
The Taqman primer probe FAM490-conjugated reagent was diluted 1 : 10 with Taqman Master Mix (both from Applied Biosystems) and 11 μl of the mix was distributed into a 96-well PCR plate.
Then, 100 µL of yeast transformation mix was distributed into each well of a 96-well half skirt PCR plate (MPX-96M2; Phenix Research Products), after which 10 µL of MoBY-ORF plasmid was added and repeatedly mixed using the BioMek FX Laboratory Automation Workstation/Biomek Software.
Similar(54)
In dPCR, the reaction mix is distributed across a large number of partitions containing zero, one or more copies of the target nucleic acid.
The average number of admissions is 1600 per year, and the case-mix is distributed into 90% of medical patients and 10% of patients requiring emergency surgery at the time of admission or during the stay in the ICU.
After mixing, the silage was distributed evenly into three 19-L buckets and sealed to provide an anaerobic environment.
After thorough mixing, the soil was distributed to common plant pots made of polypropylene (florfenicol: 9 cm width, 9 cm length, 12 cm height, 530 g soil fresh mass; tylosin tartrate: diameter 11 cm, height 9 cm, 350 g soil fresh mass).
After pooling and mixing, each pool was distributed into 1.5 ml aliquots and stored at -20°C.
Related(20)
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