Sentence examples for mix was boiled from inspiring English sources
The mix was boiled for 5 minutes then diluted with water to reach spectrophotometer linear range.
Then, the mix was boiled for 8 min at 100°C and vortexed again.
The reaction mix was boiled for 5 min and color development was monitored at 625 nm [ 29].
The mix was boiled for 10 min, to denature secondary structures, and slowly cooled to room temperature to allow primers to anneal.
The reaction mix was boiled for 5 min and the supernatant was collected and loaded on a precast 4-20% SDS-PAGE.
The reaction mix was boiled for 5 minutes prior to cooling on ice and the addition of 2.5 μl of Spectral labeling buffer (Spectral Genomics, Houston, U.S.A), 1.5 μl of either Cy3-dCTP (1mM, Applied Biosystems, Foster City, U.S.A) or Cy5-dCTP (1 mM, Applied Biosystems) and 1 μl of Klenow Fragment (BioPrime labeling kit, Invitrogen).
After the mix is boiled, it is poured through an oxtail filter and served in small porcelain cups with sugar cubes.
After mixing, the mixture was boiled for 5 min, cooled and filtrated.
Lysates or protein mixes were boiled at 95°C with 1× SDS Laemmli sample buffer, for 5 min before being subject to SDS PAGE (8 or 10% Tris glycine gel, or 4 10% gradient gels (Life Technologies) or self-prepared 16% bis-acrylamide).
