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Then 15 µl of each hybridization mix was applied to each microarray (RatRef-12 Expression BeadChips (Illumina)).
The mix was applied to RNeasy columns, and RNA purified with RNeasy Protect Kit (Qiagen) according to the manufacturers' protocols, including on-column DNase digestion (Qiagen).
Briefly, after digesting with Protease K, TdT reaction mix was applied to the cells for incubation at 37°C for 60 min, followed by incubation with streptavidin horseradish peroxidase for 10 min. The final reaction of the product was visualized by 3,3'-diaminobenzidine.
Following 20 minutes of incubation, the Lipofectamine 2000 – siRNA mix was applied to cells.
Hybridization mix was applied to sections and coverslips were sealed with rubber cement.
After denaturation at 73°C for 5 minutes, the probe hybridisation mix was applied to the smears.
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Gauze is then laid on the frame, a watery glue mix is applied on the gauze, before the rice paper is laid on and finally varnished.
To improve assay performance with fecal samples, a rapid protocol for sample pre-processing (including water-ether sedimentation and bead beating) and a modified PCR reaction mix were applied.
Fifteen cycles of PCR in the presence of dedicated PCR primers and PCR master mix were applied to generate the final libraries.
Vortex mixing was applied to accelerate the dispersion process.
In our study, nonlinear Lamb wave-mixing was applied in the detection of micro-cracks in plates.
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