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18. Prepare p-nitrophenyl phosphate solution in dH2O and mix thoroughly until fully dissolved.
Mix the coffee and sugar until fully dissolved.
The solutions were mixed, tightly capped, and left to sit until fully dissolved.
5 Stir in the sugar until fully dissolved.
Whisk in sugar and milk powder until fully dissolved.
Stir until fully dissolved and then adjust to pH 7.6 by 2N HCl).
Stir for 2 minutes until fully dissolved.
Then mix until its fully dissolved.
Stir the mixture until it is fully dissolved.
Keep stirring the mixture until the gelatin is fully dissolved.
Stir the mixture until the sugar is fully dissolved.
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