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The cards were centrifuged twice for 1 minute at 12,000 rpm to allow distribution of the reaction mix to each of the reaction wells.
A reverse transcription reaction was carried out by adding 1 µl of RT enzyme, 4 µl of Quantiscript buffer (5 x) and 1 µl of RT primer mix to each sample followed by incubation at 42°C for 20 minutes.
1.4.4 Add the Fugene/RNA mix to each well and mix gently by pipetting.
Senescent cells were stained by adding 1 ml of the staining mix to each well.
Combine components specified in Table 2. Add 4 μL of mix to each multimer for a total of 10 μL.
The microplate was kept at 37°C for 4 hours followed by adding 100 μl of the solubilization solution/stop mix to each well.
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We used 2 μl of cDNA sample in the RT-PCR mix to detect each transcript.
This OR model aims at finding the optimal cargo mix to be assigned to each ship voyage.
Superplasticizer doses were adjusted for each mix to result in slump of 100 ± 20 mm.
The amount of super plasticizer was adjusted in each mix to maintain a constant workability.
Specimens were made for each mix to quantify crack healing and to compare changes in compressive strength of concrete.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com