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For each mix, the samples were either water cured or steam cured over a 48 h period.
Within 10 min of collection, repeated inversion was used to mix the samples, which were then spun in a refrigerated centrifuge at 4°C for 20 min.
A multichannel pipette was used to mix the samples thoroughly and then they were incubated at room temperature for about 30 minutes.
Additionally, some MID-barcodes produced more reads than others even though an attempt was made to mix the samples in equimolar amounts before sequencing.
After DNA denaturation and overnight incubation with the probe mix, the samples are divided into two tubes, one of which is incubated with HhaI.
In each permutation, mix the samples in normal, CIN I and CIN III stages, then randomly select 10 samples, as well as randomly select 10 genes which are assumed to form a fictitious GO process, and calculate the Efficient Score value e of an arbitrary gene in the fictitious process.
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After addition of the probe mix, the sample was denatured and probes were allowed to hybridize.
The 30 s time interval was the time required to mix the sample, load the gel and apply the voltage.
Put 0.2 ml of suspension of cells into 10 ml PBS, and then mix the sample thoroughly.
One approach is to mix the sample with nanocolloids, but this has drawbacks associated with spectral reproducibility and sophisticated mixing schemes.
A 9-ml blood sample was collected in a polypropylene tube containing EDTA and gently inverted for a minimum of five times to mix the sample thoroughly.
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