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We benchmarked Mutascope against other mutation callers using sequencing data generated from a mixture of 8 normal DNA samples with known genotypes (MIX sample) resulting in 'somatic mutations' at variable allelic fraction.
The expression of each gene in IMB218 was calculated relative to TO1000 expression levels, and the expression of each gene in the six allopolyploids was calculated relative to the 1 to 1 parent mix sample.
Equimolar amounts of total RNA from the four species were pooled to form the MIX sample.
In the Cell Mix sample, there were initially many more P. aeruginosa cells than M. pneumoniae cells, based on the number of reads that fully mapped each species.
To estimate the impact of coverage depth, we selected reads from the MIX sample down to 50 or 10% (490 and 98×, respectively) of the maximum.
From a coriander mix sample imported from Thailand/Vietnam, a Klebsiella variicola strain (KS22), harbouring the blaOXA-181 carbapenemase gene (100%% identity after sequencing) was isolated.
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He'll perform on Tuesday night's mixed bill and Thursday morning's free "Breakfast Mix" sampler.
(c) Electrochemical impedance spectroscopy (EIS) Nyquist plot of NT, NF, and Mix samples, respectively.
Moreover, large quantities of often expensive samples are required if researchers want to mix samples by inducing turbulence.
Other instruments used were heater block with nitrogen cannula to evaporate samples, platform mixer or vortex to mix samples.
Obviously, the NT showed more effective photocatalytic activity of the unit area than the NF and Mix samples.
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