Mycelia were harvested and genomic DNA was prepared using the Kirby mix procedure [58].
Cultures were incubated at 30°C with agitation at 150 rpm for approximately 60 hr before harvested for total DNA preparation by Kirby mix procedure described in [30].
DNA was extracted using the Kirby mix procedure [ 28].
Genomic DNA (gDNA) extraction was carried out using Kirby mix procedure as described elsewhere [ 4].
Suggested further research: Investigation of the influence of mixing procedures, drum speeds, duration of mixing, time lapse between the addition of fibers and discharge, and placing methods.
To investigate any possible influence of mixing procedures, like drum speeds and duration of mixing, on fiber distribution, was considered to be outside the scope of this research work.
The influence of the mixing procedures was also investigated.
The curing regime, properties of mixture ingredients, mixing procedures and mixer type are also important parameters.
Two CNF-asphalt mixing procedures, dry process and wet process, were investigated.
Also, the effect of different fibers, mixing procedures and executive problems on asphalt concrete will be inspected.
