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Mycelia were harvested and genomic DNA was prepared using the Kirby mix procedure [58].
Cultures were incubated at 30°C with agitation at 150 rpm for approximately 60 hr before harvested for total DNA preparation by Kirby mix procedure described in [30].
DNA was extracted using the Kirby mix procedure [ 28].
Genomic DNA (gDNA) extraction was carried out using Kirby mix procedure as described elsewhere [ 4].
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Data were analyzed by ANOVA using Minitab (v.14) with the General Linear Model and Tukey Simultaneous Test; nematode demographics data for the amiRNA experiments were analyzed using a mixed model (Mixed Procedure; SAS, vers. 9.1).
The most common errors were to mix procedures NFJ52 and NFJ54.
The mixing procedure was as follows: BA and BFS were mixed for 1 min and alkali activator was then added to the dry mixture.
All mixed-model analyses were conducted using the Proc Mixed procedure of SAS, and models without random effects were conducted using the Proc GLM procedure.
To access longitudinal changes of FEV1 (often called FEV1 "slope") between the intervention and control community, we applied SAS's Proc Mixed procedure, which admits missed values, to build multilevel mixed models by adjusting for confounding and clustering effects.
Conclusion: The Super E-Mix procedure had greater sensitivity than conventional cell culture, but RT-PCR was the most sensitive technique with this type of specimen.
The analyses were conducted using the SPSS® Mixed Procedure™.
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