The detection of two individuals infected with a mix of Avipoxvirus variants 1 and 2 was unexpected given the lack of detection of genetically mixed infections in the limited number of lesion samples in a previous study [8].
The mix of these variants occurring in our in-vivo sample, could also lead to slight variations in the preferences observed as compared to results of in-vitro studies.
On the basis of a previous study (Flockerzi et al. 2008), we improved the HML-2 cDNA amplification strategy by increasing the number of amplifiable loci using mixes of primer variants considering nucleotide differences in the primer binding regions of HML-2 loci.
Variant Y93A/F233A/L367I showed varying results, thus indicating a mix of different enzyme variants.
But several experts said it was too early to know what mix of common and rare variants may cause the disease and whether that might affect the search for treatments.
The aim of this work was to investigate the acid gelation of gellan in order to gain an insight into the gel structures that can be produced by direct addition of acid, as well as investigating the effect of mixing the two variants of gellan to control the mechanical properties of the acid gel.
Then they looked at other places in the genome that influence pigmentation and found a mix of ancient and modern gene variants.
Unfortunately, reads sequenced from these types of samples often have a heterogeneous mix of various subpopulations with different variants, making assembly extremely difficult using existing assembly tools.
Thus we report a mix of FPs and "excess" positives, variants discovered with NGS that were not Sanger confirmed or not conclusively reported elsewhere.
To assess whether VHL in our validation cohort was deleted or in copy neutral loss of heterozygosity, we examined the distribution of allele fractions of heterozygous mutations in the mix of reported somatic and germline variants.
