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Two bulks of total RNAs corresponding to a mix of total RNAs from the 10 accessions of the two species were used for sequencing.
Microarray is a powerful and cheap method to identify and quantify gene expression in particular in a mix of total RNA extracted from biological samples such as the tsetse fly gut, including several organisms (here, the fly tissue and the intestinal microorganisms).
Human sperm from a male previously found to carry HPV16 in semen [13] were studied by fluorescence in situ hybridization (FISH) using biotin-labeled HPV DNA probe (a mix of total genomes containing the conserved HPV region) and visualized by fluorescence microscope (Fig. 1a).
Samples were then overlaid with 20 μL of hybridization solution containing biotin (BIO -labeled HPV DNA proBIO -labeled total genomes 7–8 Kb, containing tHPVconserveDNAprobegion) (Rembrandt in situ hybridization and detection kit HPV, Pan Path, Amixerdam, Netherlands).
This RNA is a commercially available mix of total RNA from 10 human cell lines.
An equal-molar mix of total RNA from 3 different mice was used for reverse transcription.
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Next, we formulate four scenarios describing the mix of the total electricity supply in Japan till the year 2100 corresponding to each of these nuclear power scenarios.
A mix of plasmids totaling 400 ng/µl of DNA (0, 1 mM NaHPO4 buffer pH 6.8, 5 mM KCl, 60 ng/µl helper plasmid, and generally 85 ng/µl of each of four distinct transgenesis plasmids) was injected under a Nikon Eclipse TE2000-S inverted microscope using an Eppendorf Femtojet injector and TransferMan NK2 micromanipulator.
The 10 µL of reaction contained: 2 µL of mirVana 5× RT buffer, 1 µL of miR-21 RT primer, 0.4 µL of ArrayScript Enzyme Mix, 0.2 µL of total RNA, and 6.4 µL of H2O.
Mix 1 µg of total RNA with 100 pmol of p dT 15 primer for cDNA synthesis (Roche, Hvidovre, Denmark).
Two concrete mixes with 450 and 520 kg/m3 of ordinary Portland cement and two more mixes of equivalent total cementitious materials with 30% UFGGBS replacement were cast.
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