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The gender (53.7 % female, 46%% male, and 0.3 % transgendered) and ethnic mix of the sample mirrored the university population from which it was drawn, with the majority of students reporting Caucasian (77.5 %) ethnicity, followed by Hispanic (7.5 %), Asian (5.1 %), mixed ethnicity (4.8 %), African American/Black (4.2 %), and other (0.9 %).
For instance, reflecting the area in which the research was carried out, the ethnic mix of the sample studied was very limited.
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Sample quantitation was performed using a standard curve established from a serial dilution of a mix of the samples.
Many applications need fast and efficient mixing of the sample and reagent with high throughput in a microfluidic channel.
Six consecutive cycles were run on each sample without opening the DSC furnace to ensure good mixing of the sample and reproducibility of the results.
The time and speed of mixing of the sample were chosen carefully so that shear damage in polyacrylamide samples was kept to a minimum before the experiments.
The cuvette-holder was equipped with a magnetic stirrer allowing constant mixing of the sample and a circulating water bath for temperature control.
After filling, the auto-alignment program was run for two seconds once every five minutes beginning at 4 minutes after mixing of the sample.
At the time of enrolment, 8.5 ml of peripheral blood was taken in a Becton-Dickinson Vacutainer Serum Collection Tube and gently inverted for a minimum of five times to ensure a thorough mixing of the sample.
Urine specific gravity (SG) was measured by a handheld refractometer (Atago Urine Specific Gravity Refractometer PAL 10-S; ATAGO USA, Inc., Bellevue, WA, USA) after thawing and thorough mixing of the sample with a vortex shaker.
Additionally, preanalytic factors that account for poor reproducibility, such as inadequate volume of saline in the tonometer, errors in the dwell time of the sample or in the technique used to aspirate saline, mixing of the sample with tonometer dead space and delay in analysis, could be prevented.
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