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Currently, the public sequence databases include a mix of sequences derived from type and non-type strains and with various degrees of curation and certainty.
The family-specific F-measure captures the classification quality on individual families, but does not reflect performance on a mix of sequences from families with varied conservation and architecture.
However, in the SC dataset, arthropods are represented by a mix of sequences of Drosophila and other slower evolving species when the latter have quasi-complete sequences, decreasing the global relative evolutionary distance of the OTU in the dataset.
This is because a cluster seeded with a chimera (composed of genes A and B, say) will contain a mix of sequences from genes A to B. This chimera-seeded cluster, depending on the value of k, would be able to merge with both clusters containing A sequences, and clusters containing B sequences.
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Note that some assembly programs (such as ALLPATHS-LG) expect a predefined mix of sequencing libraries as input data.
The list of top 10 features clearly indicates that features with higher classification strength are a mix of sequence, structural and physicochemical derived features.
Many of the "Other" kinase families are difficult to classify precisely; some are lineage-specific, and some have a mix of sequence features shared by multiple kinase families — the PfPK7 family, in fact, presents both problems [ 18].
For high-throughput sequencing of eleven amplified samples at a time in our laboratory, all pipetting steps are performed on a liquid-handling instrument (MICROLAB® STARplus, Hamilton Robotics) using a master mix of sequencing reagents and pre-made, single-use primer plates.
The fact that top-ranked rules are a mix of sequence and structure-based features, indicates that both groups are adding orthogonal predictive power to the model, a feat also observed in the tree models for C2 and PH domains (not shown).
Two indices, the E* and the p-score, were used to quantify the extent of the mixing of sequences from two categories in the trees (and clans).
Transient interference by siRNA was obtained by HIPK2i or Che-1i stealth RNAi sequences (a mix of different sequences) and universal negative control stealth RNAi Negative, Medium GC Duplexes (Life Technologies).
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