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Add five whole eggs to the mixer, one at a time, and mix each until yolk is no longer visible Add 1/2 cup (119 grams) of heavy whipping cream and allow mixing for approximately 1-2 minutes.
Add the rest of the sugar to the mixer and allow mixing for approximately 2 minutes.
Add half of the sugar to the mixer and allow mixing for approximately 2 minutes.
The model assumes spatially-homogeneous (age-heterogeneous) mixing in subpopulations of approximately 10 million people, which implies faster spread than more heterogeneous mixing, which we attempted to counter-balance with what might appear as a relatively low assumed rate of exportations between subpopulations.
The entire transformation mix (potentially representing a library of approximately 107 variants) was plated onto solid nutrient media containing cefotaxime (Sigma) concentrations of either 5, 10 or 20 ug/ml.
All participants had desk-based jobs and were in a mix of administrative support, teaching (approximately one-third face-to-face, two-thirds desk-based preparation) and research roles.
The reaction was then cooled in an ice bath and the contents were mixed with approximately 3 mL of a 1 1 (v/v) mixture of water:cyclohexane.
For crystallization, a variety of dHax3 (residues 231 720) and the 17-bp DNA duplex (for sequence, see Table 1) was mixed with a molar ratio of approximately 1 1.5 at 4°C for 30 min.
The RIP1 DD and FADD DD proteins were subsequently mixed at a molar ratio of approximately 1 1.
However, if they do, an outbreak can unfold very quickly in the model due to the assumption of homogeneous mixing within relatively large subpopulations of approximately 10 million people.
Briefly, serial twofold dilutions of inactivated sera (starting at 1 10) were mixed with an equal volume of approximately 30 PFU DENV1-4 and ImDENV2 in DMEM containing 2 % FBS for 2 h at 37 °C.
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