Sentence examples for mix no from inspiring English sources
For 5 μL of PCR reaction, 0.25 μL of 20x TaqMan MicroRNA Assay containing PCR primers and probes (5′-FAM), 2.75 μL of 2x TaqMan Universal Master mix, no UNG Applied Biosystemss), and 2.25 μL of RT product was mixed.
That means no potting mix, no fertilizer, no extra watering.
An absolute beginner would use a liquid malt extract to which hops have already been added, like baking brownies from a mix — no muss, no fuss.
For a perfectly dense, fudgy texture, cook until the brownie mix no longer jiggles in the centre when you gently shake the tin.
Table 2 Composition of the geoplymer mixes (Mass, %) Mix no.
Add just a little blue paint to the mix -- no more than 5-10%% of the total color at first.
Plants were seeded in 15.24 cm (6 inches) diameter pots in standard potting media (Seedling Mix No. 1, OBC Northwest, Canby, OR) and cultivated in greenhouses at 20 30°C temperature and 16/8 hr day/night light regime.
Primers and probes were mixed with TaqMan® Universal PCR Master Mix, No AmpErase® UNG (Applied Biosystems, Foster City, CA, USA) and added to 96-well microtitre plates, each well of which contained 10 ng of air-dried DNA.
Reaction mix consisted of 10 μL 2 × TaqMan Fast Universal PCR Master Mix, No AmpErase UNG, 1 μL 0.2 μM TaqMan probe, 3 μL 1.5 μM of forward primer, 1.4 μL 0.7 μM reverse primer, and 1.33 μL of cDNA.
For each RT pool, 1 μg of total RNA was used and the product was diluted 1 75 and mixed 1 1 with TaqMan® Universal PCR Master Mix, No AmpErase® UNG.
