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Dissociated hNSCs were plated at a density of 106 cells/ml in control (media/hormone mix) medium devoid of any growth factors and supplemented with 1% fetal bovine serum (FBS) on poly-L-ornithine-coated (15 mg/ml; Sigma) glass coverslips in 24-well Nunclon culture dishes with 0.5 ml/well.
When glycerol in the xylose/glucose mix medium was added, the maximum TAG yield per carbon source consumed was higher than that with the xylose/glucose mix alone.
These CHO cells were grown and maintained in F12 nutrition mix medium with 10% fetal bovine serum (Invitrogen) at 37°C and 5% CO2.
From day 2 to 8, the cells were grown on F15 medium supplemented with 2 mM valproic acid (VPA, Sigma-Aldrich), whereas, from day 9, F15 K15 (1:1 mix) medium lacking VPA was used.
The cell pellet was resuspended in SelenoMet Plus Nutrient Mix medium containing ampicillin, and the incubation was continued at 37 °C for 1 h to deplete the intracellular methionine stock.
This starter culture was used to inoculate a larger culture with 100-fold dilution in SelenoMet Plus Nutrient Mix medium supplemented with 1 mM methionine and 100 μg/mL ampicillin.
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Mr. Charles told The Hollywood Reporter that the show would try to depict "contemporary American life through the prism of a do-it-yourself homemade aesthetic that will mix mediums".
The world of Off Off Broadway is a welter of scrappy companies fighting for attention, producing when and where they can, and forsaking linear storytelling and text-based work in favor of productions that mix mediums more freely.
If you're using glitter, you should use a mixing medium.
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Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com