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This exhibition compiles further efforts to mix life and art, albeit in a more crowd-friendly way.
PCR was conducted using the Power SYBR green master mix (Life technologies) following manufacturer's instruction.
Real-time PCR detection with SYBR Green Mix (Life technologies) for the targeting genes was described previously [ 16, 18].
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Second strand master mix (Life Technologies) was then used to synthesize the second-strand cDNA.
For labeling of cells by GFP, pLenti-CMV-EGFP plasmid was transfected into 293T cells together with lentivral packaging mix (Life Technologies, USA).
The reactions were run in duplicate using Fast SYBR Green Master Mix (Life Technologies, Carlsbad, CA, USA), according to the manufacturer's instructions.
qPCR was performed using Power SYBR Green PCR Master mix (Life Technologies).
Then, RT products were amplified with TaqMan PreAmp master Mix (Life Technologies), when necessary.
For TaqMan analysis, reverse transcription was carried out using the High Capacity RNA-to-cDNA Master Mix (Life Technologies).
The PCR amplification was performed using Fast SYBR green master mix (Life Technologies) and StepOne Plus (Life Technologies).
Quantitative real-time PCR (qPCR) analyses were performed using the SYBR Green master mix (Life Technologies, Courtaboeuf Cedex, France).
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CEO of Professional Science Editing for Scientists @ prosciediting.com