Your English writing platform
Discover LudwigExact(33)
Real-time PCR reactions were performed in duplicate, using 'SYBR® Green PCR Master Mix' kit (Applied Biosystems , Inc.
TaqMan one-step master mix kit (Applied Biosystems) and gene-specific target primers and probes were used for amplification.
The amplification product was introduced into plasmid pMT30 by recombination using the In Fusion Dry and Down Mix kit (Clontech).
RNA samples were subjected to qRT-PCR, and reactions were done in duplicate using a TaqMan One-Step PCR Master Mix kit.
Real-time RT-PCR was performed using the SYBR-Green PCR Master Mix kit (Bio-Rad, USA) in the Light Cycler (Bio-Rad, USA).
A multiplex preamplification step was used to increase the quantity of specific cDNA target (TaqMan Pre-Amp Master mix Kit, Applied Biosystems) following manufacturer's instructions.
Similar(24)
For the TaqMan assay, one-step RT-PCR Ready-Mix Kit (Applied Biosystems) was used.
Relative gene expression was determined using a peqGOLD Hot Start-Mix kit (Peqlab) and a StepOne real-time thermocycler (Applied Biosystems) according to the manufacturer's instructions.
The Fast-Run Taq Master Mix kit was purchased from Protech Technology Enterprise (Taipei, Taiwan).
qPCR was performed using the AceQ qPCR SYBR Green Master Mix kit (Vazyme Biotech, Nanjing, China) according to the manufacturer's instructions.
Real-time PCR was performed using the 7500 real-time PCR system (Applied Biosystems, Foster City, CA, USA) and Quantitect SYBR Green Master-mix kit (Qiagen).
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com