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The results suggest that the concrete mix containing the manufactured plastic aggregate at a replacement level of 25% can be used in structural and non-structural applications requiring moderate strength and ductility.
As binder, ordinary Portland cement (OPC), ground granulated blast furnace slag (GGBS) and pulverised fuel ash (PFA) were used to produce different concrete mixes: OPC, GGBS and ternary mix containing the three binders, respectively.
The cDNA was diluted and added to a master mix containing the fluorescent SYBR Green dye.
When needed, biotin-labeled mAb against TCR were used alone, followed by a mix containing the FITC-labeled streptavidine and the other mAbs.
MGC cDNA clones were screened by pre-spotting 40 ng/well cDNA into Greiner white solid bottom 384-well tissue culture plates, then addition of transfection mix containing the DMD-Luciferase reporter gene construct +/−AON.
A master mix containing the CTCFL probemix (Table S2), DQ- and DD-control probes (MRC-Holland) and MLPA buffer were added and the mixture was heated briefly (1 min).
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To minimise nonspecific product formation, the reaction Master Mix contains the enzyme AmpliTaq Gold® DNA polymerase.
The PCR master mix contained the two upstream primers, the downstream primer, and two probes in concentrations described previously [ 23].
The PCR mix contains the primers, which bind either side of the multiple cloning site and thus allows detection of cDNA fragment sizes.
Briefly, the enzyme mix contains the bacteriophage lambda recombination proteins Integrase (Int) and Excisionase (Xis), and the E. coli-encoded protein Integration Host Factor (IHF).
A PCR master mix contains the THUNDERBIRD Probe qPCR Mix (Toyobo, Japan) with specific primers (SS-F4 and LTR Rev) as previously described above and probe: SupINprobe (5′-6FAM-CTggTACCTAgAgATCCCTCAgAC-BBQ-3′) at final concentration of 175 nM.
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