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The Ipomoea mix contained a concentration of LSA about 10 times lower than that of seized Morning Glory seeds.
The Ipomoea mix contained a concentration of LSA about 10 times lower than that of Morning Glory.
The seed mix contained a mixture of seeds, shell grit and essential vitamins and minerals.
The reaction mix contained a total volume of 20 µL and the thermal cycling consisted of UDG incubation at 50°C for 2 min, 40 cycles of 95°C for 15 s and 60°C for 33 s for amplification.
The antibody mix contained a set of lineage specific surface markers found on mature hematopoietic cells; CD2, CD3, CD14, CD16, CD19, CD24, CD56, CD66 and glycophorin A. Following isolation, the Lin- cells or the Lin+ cells were used directly in the SRC-assay.
Each reaction mix contained a 15 ng RNA equivalent of cDNA and 10 μM gene-specific primers.
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A remix of "2 Bad", is featured on Blood on the Dance Floor: HIStory in the Mix containing a sample of "Beat It" as well as a rap by John Forté and guitar solo by Wyclef Jean.
The EGFR p.L858R mutation was detected using a probe mix containing a wild-type probe and two different mutant probes: one for the most common variant (c.2573T>G) and one for the rare complex c.2573_2574TG>GT inversion.
Reverse transcription (RT) lasted for 2 hours at 50°C using a master mix containing a 4 1 ratio of aminoallyl-dUTP to TTP.
The restriction product was then added to 1× ligation buffer, T4-ligase (New England Biolabs® Inc., Frankfurt, Germany) and an adapter mix containing a common adapter and a barcode adapter unique to each sample.
To set up this experiment, we used ERCC RNA spike-in control mix as our sample, because each mix contains a defined number of copies for each RNA transcript [ 21].
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