1.25 µl of cDNA from each sample was mixed with master mix consisting of 5 µl Cells Direct 2X reaction mix (Invitrogen), 2.5 µl 0.2X PPP mix (48 genes, Taqman/Applied Biosystems Inc, Foster City, CA, USA, http://www.appliedbiosystems.com), 0.5 µl Platinum Taq (Invitrogen) and 0.75 µl TE Buffer.
Samples in lysis buffer were mixed 1 : 1 with 50 μl caspase-3 substrate mix, consisting of 9.8 μM DTT and 49 μM Ac-DEVD-AFC (Invitrogen).
Most ice cream manufacturers make a standard mix consisting of milk, cream, sugars, and stabilizers, to which flavours may be added just prior to freezing.
An electricity mix consisting of a larger share of zero-emission technologies was selected, that of Switzerland.
A technology mix consisting of digitalized documents, the detection of geographic positions via telematics, special onboard terminals for status monitoring and an integrated flow of information via the Internet support these companies in delivering more efficient services.
The washed spore mix, consisting of 95% BS and 5% SCS, as well as pure AC from a two-weeks-old SDA culture were suspended in 0.1% Triton-X114 to obtain a final concentration of 106 viable spores/mL.
After confirmation of correct size product, students loaded five μl of a master mix consisting of two μl of Exonuclease I and three μl Shrimp Alkaline Phosphatase (EXO/SAP Thermo Scientific Fermentas) to each tube.
Model tars included benzene and toluene representing the simplest and most predominant of biomass gasification tars, and a tar mix consisting of higher molecular weight tars such as naphthalene, pyrene, and phenol.
A reaction mix consisting of 29 µl reaction buffer, 10 µl water, and 1 µl enzyme was prepared on ice, and then added to the denatured template.
Plasmid DNA including non-targeting shRNA as control (sh-control) was transfected into MH14 cells along with Lentiviral Packaging Mix consisting of an envelope and packaging vector (Sigma-Aldrich) to produce lentivirus packed with shRNA cassettes using the standard procedure.
Reaction mix consisting of 50 μl of reaction buffer +50 μg/ml of substrate (FITC-gelatin) was added to the section for 24 h in a wet darkroom, at 37°C.
