The soil mix consisted of a 2 1 1 volume of silt loam (Vienna fine-loamy mixed Udic Haploboroll), sand, and peat, respectively, hereafter described as soil.
The concentrate mix consisted of soybean meal, canola cake, and beet pulp.
The 25 μL RT-PCR reaction mix consisted of 1 μL of eluted RNA, 14 μL of RNAse-free water, 5 μL of RT-PCR buffer (5X), 1 μL of dNTP mix (10 mM), 1.5 μL of each primer (10 μM) and 1 μL of RT-PCR enzyme mix.
The mix consisted of three chemicals common to many commercially available herbicides: 2,4-D, mecoprop, and dicamba.
The F3B mix consisted of Canadian sphagnum peat moss (50%%), processed pine bark, perlite, vermiculite, starter nutrients, wetting agent and dolomitic limestone.
The reaction mix consisted of 5 µL of master mix (Roche, Germany), 1 µL of each primer (1 µM final), 1 µL of molecular grade water and 2 µL of cDNA.
The concrete mix consisted of crushed dolomite, sand, and Portland cement with coarse to fine aggregate ratio of 2 and sand to cement ratio of 2. The water/cement ratio was 0.4.
Since no further mortality tests were conducted with pure BS and pure SCS, it can only be hypothesized that BS must have killed the larvae, because the spore mix consisted of 95% BS which are the preferred propagule of B. bassiana in the haemocoel of infected insects (Jackson et al. [2010]; Shimizu et al. [1993]; Sieglaff et al. [1997]).
The reaction mix consisted of 5 μL standard/diluted cDNA template (1/10 to 1/1000), 0.5 μL 10× primer for the gene [final 0.5 μM primer, and 2.5 4 mM MgCl2], 2 μL water, and 2 μL FAST-START DNA Master SYBR Green I mix (Roche).
The second mix consisted of 2X divalent cation (MgCl2) in the same buffer and was loaded in the second syringe.
Transfection mix consisted of 2 µl siRNA (50 µM), 10 µl lipofectamine2000 (Invitrogen) and 588 µl OptiMEM (Invitrogen), with a final volume of 500 µl.
