Exact(8)
When growing bacterial cultures, biologists often mix cells in with nutrients in one big jar, then swirl, much like an aficionado over the latest vintage.
We hypothesized that the high viscosity of pre-gelled solutions typically used in these applications may decrease cell viability due to the high shear forces required to mix cells with these solutions.
However to date, the majority of experiments have been performed using α-factor administered in a spatially uniform fashion (i.e. mix cells and pheromone together in a tube).
After adding the mix, cells were incubated for 24 h.
After an overnight incubation in the transfection mix, cells were washed and fed with EGM-2MV.
Prior to the addition of transfection mix, cells settled in the plate were washed three times with max-XP serum-free Insect cell medium (BD Baculogold).
Similar(52)
Mix cell suspension.
At all times, it was necessary to thoroughly mix cell pellets with a Pasteur pipette prior to counting in order to avoid large cell clusters.
HPLC grade water and acetonitrile were purchased from Fisher Scientific (Fair Lawn, NJ), and BugBuster master mix cell lysis buffer was purchased from EMD Millipore (Novagen, Billerica, MA).
Most late primary cultures present a fluorescence profile covering both intensities, indicating a mix cell population resulting from the polyclonal origin of the cultures.
Similar to the induction of mN1IC, Jagged1 accelerated myeloid differentiation of 32D and FDCP-mix cells.
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