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In Drosophila it appears to be essential for the coordination of mitosis in the syncytical embryo as in mcph1 mutant flies mitotic entry is slowed with prolonged prophase and metaphase stages, while centrosomes are frequently detached and prematurely separated.
Furthermore, in Figure 4, data on mitotic entry is lacking.
The importance of CUL3-KLHL18 in mitotic entry is consistently evident in our data.
The result is added to Figure 4. Furthermore, in Figure 4, data on mitotic entry is lacking.
In summary, inactivation of inhibitory kinases advances mitotic entry at low CycB level, and mitotic entry is still irreversible.
The accumulation of CycB pulls the switch when the threshold for mitotic entry is reached at the G2/M transition.
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The morphological alterations occurring during mitotic entry are tightly coordinated with the cell cycle, mainly through the action of mitotic kinases.
Consequently, timing of mitotic entry was monitored over two periods of cell division.
Cdk1/cyclin B activity and mitotic entry are further controlled by additional superimposed feedback mechanisms upregulating the mitotic kinases Plk1 and Aurora A, and the Aurora/Plk1 activator Bora, coupling mitotic entry to centrosome maturation, and stimulating expression of proteins of the mitotic entry network, such as cyclin B [4].
Time-lapse microscopy and other approaches indicated that mitotic exit but not mitotic entry was delayed.
Mitotic entry was significantly delayed in CUL3 depleted cells compared to control cells.
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