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The UK government's decision this February to allow mitochondrial substitution in the clinic to prevent embryos developing with mitochondrial diseases, a form of germ-line therapy, was premised on the basis that the small amount of DNA mitochondria contain is found outside the cell nucleus.
We found that by treating N. jurassica as stem Nephila or nephilid the inferred clade ages were vastly older, and the mitochondrial substitution rates much slower than expected from other empirical spider data.
We showed that mitochondrial substitution rates of Castor differed significantly from those of the other rodents in the dataset.
The implementation of a relaxed molecular clock enabled us to estimate mitochondrial substitution rates and to evaluate the effect of life history traits on it.
In our analysis of mitochondrial genomes, body mass and age at sexual maturity did not correlate significantly with mitochondrial substitution rates, although body mass did so earlier [21].
As beavers are the largest rodents for which a mitochondrial genome sequence is currently available, we explored the mitochondrial substitution rate in comparison to other rodents.
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The mean ratio of this sample is 5.28 (+/− 3.87) chloroplast:mitochondrial substitutions.
For example, Rand et al. (2004) demonstrated that the association between nuclear and mitochondrial substitutions drives the evolutionary divergence [ 105].
The single variable regressions, summarised in Table 1, show that of the four variables tested, body mass and maximum lifespan are individually significant predictors of mitochondrial synonymous substitution rates: Species with greater mass, or longer lifespan tend to have slower rates of synonymous substitution.
We find that mitochondrial synonymous substitution rates, estimated from the 9 longest mitochondrial genes, show strong negative correlations with body mass and with maximum recorded lifespan.
Silene noctiflora (Caryophyllaceae) is a recent addition to a growing list of angiosperms exhibiting major accelerations in mitochondrial synonymous substitution rate [ 4, 7].
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