A small number of specimens were frozen for mitochondrial separation procedures (see below).
Mitochondrial separation medium (215 mM mannitol, 75 mM sucrose, 0.1% bovine serum albumin, 20 mM HEPES, 1 mM EGTA, adjusted to pH 7.2) was added at volume ratio 1 : 10, and 10 rounds of homogenization were performed (six with tight fitting pellets and four with loose fitting pellets) to ensure that no chunks of brain tissue remained.
Reinders, J., Zahedi, R. P., Pfanner, N., Meisinger, C. & Sickmann, A. Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics.
For mitochondrial and cytosolic separations, we used the mitochondrial isolation kit for cultured cells (Pierce), according to the manufacturer's instructions.
As can be seen in figure 2A there is substantial separation of mitochondrial (red) and non-mitochondrial (green) diseases using this approach.
The other grass proteins included in the phylogenetic analysis are more similar to mitochondrial AtGPx6, indicating that separation of the AtGPx6 lineage and the AtGPx1/AtGPx7 branch took place prior to separation of monocots and dicots.
Molecular analysis of mitochondrial DNA shows a separation of the species into two clades, an Old World alba and a New World furcata, but this study did not include T. a. delicatula which the authors seem to have accepted as a separate species.
Effective separation of mitochondrial and nuclear nucleotide substitutions is an intricate process due to the absence of apparent criteria.
and performed western blot analysis of C. elegans homogenates after separation of mitochondrial and cytoplasmic fractions (see Mat. & Meth).
Accordingly, mitochondrial fission allows the separation and elimination of seriously damaged organelles by autophagy [11], [39], [40], [41], while fusion enables the exchange of mitochondrial compounds.
For separation of mitochondrial and cytoplasmic fractions, worms were harvested from well grown NGM plates and washed twice with M9 buffer.
