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Far from explaining the elevated level of ATP in TER cells, indications that FIBs bore a larger mitochondrial complement than TER cells argued that FIB/TER differences were likely to reflect some form of improvement in mitochondrial quality as opposed to quantity.
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The data we report confirms earlier studies that IPSCs display a mitochondrial complement that, in most respects, does not differ significantly from the mitochondrial complement of natural human ESCs.
Mitochondrial membrane potentials of all cell types were analyzed using flow cytometry and the vital dye JC-1[24] to further study the mitochondrial complement.
As stem cells of the early embryo mature and differentiate into all tissues, the mitochondrial complement undergoes dramatic functional improvement.
With the establishment of differentiation in the developing embryo, the mitochondrial complement both diversifies and amplifies depending on the cell type.
This further indicated that the TER cell mitochondrial complement differed significantly from the input parental fibroblasts as well as the pluripotent cell types.
A high-quality oocyte mitochondrial complement is critical to provide the best possible start for the generation of a completely new individual[18].
Such a sequence is expected to arise following inter-molecular maxicircle recombination, which necessitates the inheritance of mixed mitochondrial complements.
The typical mitochondrial gene complement encodes 13 protein subunits of the oxidative phosphorylation enzymes, 2 rRNAs and 22 tRNAs.
With minimal exceptions, the mitochondrial gene complements of eukaryotes are subsets of the superset of 66 genes found in jakobids.
Mosquitoes from Bangkok thus displayed higher mitochondrial diversity than those from Cairns (Fig. 4).
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