Sentence examples for mitochondrial cluster from inspiring English sources
The oocytes were examined for nuclear maturation, mitochondrial cluster formation, lipid consumption, fertilization and embryo development.
However, to our surprise, unlike the clusters induced by the four proteins above, here, the mitochondrial cluster induced by overexpressing APEX2-Mfn2 was relatively loose, especially at the inside region that was filled with strong staining signals.
Through investigation of the staining patterns in the 3D volume data, we found Mff and hFis1 distributed widely around the surface of mitochondrial outer membrane, and they were consistent with MiD51/49 except that the staining signals of MiD51/49 were reduced at the periphery of mitochondrial cluster.
In addition, through investigation into the 3D volume of the clustered mitochondria, we found that Mfn2 was only located in the contact sites of clustered mitochondria, and no staining signal was found at the periphery of mitochondrial cluster (Fig. 3E; see also Movie S5).
Through investigation of the 3D volume of the clustered mitochondria (Fig. 3A and B; see also Movies S1 and S2), we clearly found that the target proteins MiD51/MiD49 were largely localized at the interface among mitochondria with reduced amount of proteins surrounding the peripheral of the mitochondrial cluster.
Through investigation of the 3D volume of the clustered mitochondria, we also found that hFis1 was located in high density at the interface of clustered mitochondria with a significant amount at the periphery of mitochondrial cluster (Fig. 3D; see also Movie S4).
However in comparison with the untreated controls, the proportion of MII oocytes with mitochondrial clusters was significantly higher only in the l-carnitine-treated MLC oocytes, which also showed a significantly lower mean lipid content.
Additionally, we estimated the number of mitochondrial clusters based on a novel single-locus delimitation method and the time of their divergence, using recently inferred evolutionary rates for lacertid species.
This observation implied how MiD51/MiD49 induced mitochondrial clustering by recruiting Drp1.
Open image in new window Figure 8 Trak1 overexpression causes mitochondrial clustering in mitofusin-deficient cells.
(K) Mitochondrial clustering was analyzed by categorizing cells based on the criteria of complete clustering, partial clustering, or no clustering.
