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Mitochondrial amount was determined by the DNA copy number.
The C2C12 cells were examined for oxygen consumption rate, mitochondrial amount and gene expressions.
The mitochondrial amount in the skeletal muscle was increased with the low dose and standard dose (Fig. 2C).
Full-length blots are included in the Supplementary Information, (P) Mitochondrial amount in various tissues (WAT, liver, kidney and heart).
In cultured myocytes, ALA increased the expression of PGC1α and mitochondrial amount and elevated the oxygen consumption rate.
The mice that received ALA for 8 weeks gained muscle strength and endurance, and exhibited increased muscle mass and mitochondrial amount.
Similar(41)
Optimization of synaptic mitochondria demonstrated that mitochondrial amounts greater than 10 μg per well have a non-linear state 3 rate (Supplemental Figure 2A), therefore we used 10 μg of mitochondria for subsequent experiments.
They may be caused by mtDNA mutations, nuclear gene mutations (for example in genes encoding subunits of respiratory chain complexes), or by mutations in nuclear genes encoding proteins involved in mtDNA replication and stability, having a secondary effect on mitochondrial DNA amount and quality (Spinazzola and Zeviani 2007).
The resulting embryo carries DNA from its parents as usual, but its mitochondrial DNA – amounting to 0.2% of the total – comes from the donor.
Analysis of relative mitochondrial ATP amounts using COX-8 luciferevealedealed that the mitochondrial ATP level was significantly reduced in LETM1 overexpressed cells compared to control (Fig. 1C), indicating that LETM1 inhibited the production of mitochondrial ATP.
The width of the IMM separating the regions with dome- or triangular-like profiles, as measured from the mitochondrial matrix, amounted to ∼110 and 250 nm, respectively.
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