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e The ratio of Drp1 expression in mitochondria and cytoplasm were performed by densitometric analysis.
Algae and plant cells have subcellular compartments such as chloroplast, mitochondria, and cytoplasm.
Cytochrome c oxidase subunit IV (COX IV) and β-actin were used as a mitochondria and cytoplasm marker, respectively.
b The relative levels of fission proteins and fusion proteins expression in mitochondria and cytoplasm were performed by densitometric analysis.
Similarly, Tay and coworkers found that ~2 nm Au nanoclusters capped with mercaptopropionic acid increased ROS product in both mitochondria and cytoplasm, but it did not lead to any detrimental cellular effects in human derived colonic epithelial NCM460 cells [57].
A critical enzyme in counteracting the toxicity of these species is superoxide dismutase, which is present in both the mitochondria and cytoplasm of eukaryotic cells.
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The subcellular organelles, i.e., nuclei, mitochondria, microsomes, and cytoplasm, were isolated by centrifugation and verified for their authenticity by determining the markers specific to cellular organelles.
These enzymes are distributed in mitochondria, peroxisomes, and cytoplasm.
These NAD+-dependent protein acetylases are localized in the nucleus (SIRT1, SIRT6 and SIRT7), mitochondria (SIRT3-SIRT5) and cytoplasm (SIRT2) [ 88- 90].
However, both concentrations of Cr VI reduced the labeling highly significantly in mitochondria, chloroplasts and cytoplasm (Fig. 7A and B).
The results were consistent with the three hypothesized sources of lipids and proteins for the creation of peroxisomal membrane namely mitochondria, ER and cytoplasm.
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