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Even a conformationally homogenous specimen would show a similarly smooth distribution (but perhaps with a slower falloff) given the nature of cryoET data: 1) The raw data (individual TRiCs) are very noisy and 2) the missing wedge affects individual raw particles in different ways.
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All tomograms are affected by missing wedge artifacts.
Note that the tomograms shown in (I and L ) are obviously affected by missing wedge artifacts, whereas the tomogram in (F ) (long axis of the molecule parallel to the beam axis during tilting) is only slightly stretched in comparison to the original model (C ).
In the first case, the complexes were strongly affected by the missing wedge.
However, we are aware that these tomograms are still affected by the missing wedge along the z direction, as pointed out by the reviewers.
DOI: http://dx.doi.org/10.7554/eLife.03600.008 The orientation of our crystals prevented the full sampling of reciprocal space leading to systematic incompleteness (missing wedge or missing cone).
The potential artifacts that are caused by the missing wedge in the EM data could have affected this particular conclusion, especially the connections between the filaments, as the authors also showed in their simulations.
Despite the recent progress in the field of reconstruction and regularization techniques, the missing wedge artefacts remain untreated in most current reconstruction workflows in structural biology.
Fig. 5 Missing wedge reconstruction, processed with NAD. a A WBP reconstruction of aligned, noiseless projections with a 28 ° missing wedge.
Fig. 10 Missing wedge reconstruction processed with Weka. a The perfectly aligned, noiseless 28 ° missing wedge reconstruction from Fig. 5. b The same reconstruction but processed with Trainable Weka Segmentation.
Hessian, Gabor and structure filters detect the orientation of image features one very specific usage case for these is when compensating for missing wedge artefacts.
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