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To estimate the completeness of the networks, the missing relationships of non-predicted essential genes in the KEGG2path network were determined by grouping all essential genes by their Gene Ontology biological process ID.
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The temporal relationships between the induction of the different markers was variable between individual subjects, and using set days may miss relationships where timing of events varied.
A detailed analysis of data showed that ANOVA was only partly permissible due to not normally distributed data, considerable differences in variance and type of distributions to be compared, or missing linear relationships of parameters.
Then, the missing local relationships of unpredicted essential genes in these groups were determined.
To determine if there are missing genetic relationships in our REMG networks, we performed two analyses: i) the identification of known metabolic genetic relationships (see Methods) in our reconstructed networks and ii) the identification of the missing local relationships of non-predicted essential genes (see Methods).
In order to avoid missing biological relationships because of the arbitrary nature of the cut-offs used to generate gene lists, we compiled additional lists of up and down-regulated genes for each of the EBNA3 knockouts, using more stringent p-value criteria (<0.0001) for smaller fold-changes (>1.3).
It has been shown that essential genes tend to be part of Essential Complex Biological Modules [ 21]; in that case, the missing local genetic relationships of unpredicted essential genes may be found within these modules.
And now I was missing that relationship emblematic of my proximity to Fred, a person I knew and respected merely because of who he was, not what he was.
Another limitation is the missing analysis of possible relationships between smoking, alcohol and weight status.
If the dialogue is missing, the relationship is one-way instead of mutual.
The missing linear relationship across the whole range of RBC's age is biologically plausible given the possibility of a maximum level of deleterious changes in RBCs over time.
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