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In contrast, the CV2 scheme investigated in the current paper may be used to predict missing phenotypes caused by random missingness for a same set of genotypes evaluated across multiple environments.
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In the second strategy, 1,600 cows had complete information (genotypes and phenotypes) and 400 cows were genotyped, but with missing phenotypes for all THI.
Eight animals were removed because of missing phenotypes.
A missing genotype was coded as 0. The data contained about ∼4.9% missing marker genotypes and a few missing phenotypes.
The animal model implicitly assumes that missing phenotype observations are missing at random, and hence we (implicitly) have assumed this.
Two additional individuals were removed due to missing phenotype data, leaving 1,016 individuals for association analysis.
In total, 5049 individuals were analyzed after excluding those with missing phenotype.
For shg RNAi in muscle we observed a flightless phenotype caused by missing indirect flight muscles in the thorax [8].
Knockdown-resistant isoforms of Dync1i1 can partially rescue the phenotype caused by shRNA1-6 (Fig. S1C H).
We hypothesized that the missing posterior Eve expression and accompanying loss of posterior larval segments could either be an effect of direct regulation of Eve by the endo-siRNA pathway, or an indirect phenotype caused by an inability to correct for differences in developmental time in the two halves of the embryo, which are at different temperatures.
The phenotype is a milder SBBS phenotype caused by haploinsufficiency.
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