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Most samples had very low rates of missing data, and we removed 58 individuals with ≥ 10% missing genotypes, leaving 943 for further analysis.
From the 4,336 participants 106 (2.44%) were excluded due to >50% missing genotypes, leaving 4,230 participants for further analyses.
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Of the 768 SNPs genotyped, 29 were excluded because they were not polymorphic, or had heterozygous or missing genotypes for many samples, including parent strains (Fungal Genetics Stock Centre A4 and A91), leaving a total of 739 markers.
SNPs with missing genotypes (>5%) and with minor allele frequency (MAF) <5%, and/or violating Hardy Weinberg equilibrium (p < 0.001) were excluded, leaving a total of 301 SNPs for association study.
Markers with MAF <1%, SNPs with >5% missing genotypes and markers that failed an exact test of HWE (P < 5 × 10−6) were excluded from further analyses leaving 310,505 SNPs that passed quality control.
The performance of the genotyping assay for each method was assessed using three criteria: missing genotypes (assay fail rates), incomplete genotypes (loci departing from HWE) and inaccurate genotypes (inconsistent genotypes across replicate samples).
This has resulted in high rates of missing genotypes (assay fail rates of 21%), incomplete genotypes (64% of loci departing from HWE) and inaccurate genotypes (1% of genotypes inconsistent across replicates).
SNPs with ≥2 missing genotypes were excluded.
The overall rate of missing genotypes was 9%.
Based on estimated parameters, missing genotypes are inferred.
Samples were screened for the proportion of missing genotypes, and animals with greater than 10% missing genotypes were removed.
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