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Moreover, using non-normalised cDNA libraries likely resulted in missing genes with low expression levels.
Whole genome tiling arrays provided experimental evidence for missing genes, with the detection of signals "intergenic" to OGSv1.0 genes [ 1].
Future research on myopia will be focused on finding the missing genes with exome analyses, whole genome analyses, and family studies; on pathway analysis and functional studies; and on development of prediction models.
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But organise themselves they do—unless, Dr Cattaneo found, they lack huntingtin.Replacing the missing gene with its equivalent from another species, however, restored the cells' ability to organise themselves.
However, this method may still miss genes with low expression levels that could be important in pathogenesis, although such genes would seem less likely to make good biomarkers.
With this method, we found a varying number of missed genes with potential functions (Table 3).
We could associate 1,264 missed genes with phenotype data stored uniquely in COMBREX.
By the end of our search for candidate missed genes, we found 13,602 named missed genes with significant sequence similarity to a known gene with non-hypothetical annotation, and 39,003 hypothetical missed genes with homology only to hypothetical proteins.
Alternatively, maSigPro can miss genes with less pronounced changes, which can be recovered by ASCA-gene if their profile is abundant within the dataset.
For those candidate missed genes with homology only to hypothetical proteins, we needed additional information to determine if they were indeed genes.
We note that the reciprocal best-hit approach is prone to miss genes with high sequence divergence, including those that underwent particularly intense divergent adaptive evolution.
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