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On the other hand MEP and Pathway Tools hole filler represent an alternative approach that tackles the gap filling issue identifying missing genes rather than missing reactions, and these tools achieve this goal using expression data and species homology, respectively.
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Many of the missing genes likely reflect incomplete genomes rather than deletions.
Reassuringly, we found that most annotation disagreements were due to a missing gene-reaction relationship rather than a contrasting association; this suggests that overlapping reactions comprise a well annotated area of the network.
So where are the missing genes?
This can explain some missing genes.
Dr. Fischer said he began working on gene therapy for SCID-X1 in 1993, as soon as the missing gene was identified.
Given that GS 20, and by extension GS FLX, pyrosequencing is known to provide good coverage of genic regions [ 24], these gaps likely represent repeat regions rather than missed genes.
Even though a small number of missed genes may be overlooked by our approach, our goal with this study was not to find all missed genes, but rather a large subset thereof that could be easily found through existing tools.
The remaining candidate missed genes were termed hypothetical missed genes.
Functions of the missed genes.
In summary, although genes missing from GenBank are not necessarily missing from RefSeq, a sample of missed genes from GenBank we found were also missing from RefSeq.
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