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Normalized microarray data were further filtered for missing genes and for genes exhibiting low expression levels.
Normalized microarray data were further filtered for missing genes and for genes with low expression levels.
There are several possible sources for the missing genes, and this is a subject of intense argument and ongoing research.
MPS studies on larger numbers of DSD patients will help identify the missing genes and networks, which, when disrupted, cause DSDs.
If genes are missing or poorly annotated in the reference GenBank file, the Plann output will also be missing genes and be poorly annotated.
After each step in the automated definition pipeline, the presence of the manually defined genes was checked, allowing troubleshooting of missing genes and improving our pipeline.
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Proteogenomic analysis confirmed the quality of annotation and improvement pipelines, identifying a missing gene and an alternative reading frame.
In this first matrix, 44 of the ingroup taxa were represented by all eight genes used in the analyses, four taxa had one missing gene, and one taxon had two missing genes.
Actually, how well the estimator works directly depends on the number of observed entries in the missing gene and the correlation information between conditions.
GCM2 is homologous to the Drosophila glial cells missing gene and encodes a transcription factor implicated in parathyroid gland development, and mutations in GCM2 have been reported in familial hypoparathyroidism [ 28, 29].
We suggest two possibilities for the missing genes: 1) Either the missing genes were recently lost, in which case the pathway is no longer intact and we would expect the remaining genes with no other roles will eventually accumulate mutations and be lost, or 2) a diverged homologous gene or a nonhomologous neofuncionalized gene may take the place of the missing gene and keep the pathway intact.
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