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Prior to analysis, the dataset was analyzed for missing data (see Table 1 for variable level missing data percentages).
The criterion we will use is the maximization of the conditional expectation of the joint likelihood of both observed samples,, and missing data, (see [11] for details about this criterion).
As a result, the different sequences do not exhibit similar lengths, resulting in a substantial amount of missing data (see Figure S3 for an alignment of polymorphic sites).
Based on the lack of behavioral data (scored as missing data, see also [21]), the presence of PSD in Clitaetra and Herennia (Nephilidae) as well as in the closest related taxa Argiope (Araneidae) is ambiguously optimized and should be addressed in future studies.
For missing data, see text.
c Markers with more than 70% of missing data (see Methods section) and identical markers.
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The rates of missing data seen for items related to sexual intercourse ranged from 4.7%to90.6%6%.
Variscan is able to handle the large amount of missing data seen for the SGRP data, in estimating levels of polymorphism.
This was done to mimic the pattern the missing data seen in our cohort where question 6 was found to be missing approximately twice as often as other questions among the incomplete cases.
The number of saccades for trials with missing data points (see above) was corrected by a mean imputation of saccades of the respective trial.
The type I error rate still remained stable and close to the expected value (5%) for all methods, whatever the proportion of missing data generated (see Table A3 in Additional file 3).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com