Given the risk of missing data or insufficient image quality, this sample size was extended to at least 60 patients.
It was not possible to include these patients in the analysis, even using statistical imputation methods to account for missing data, as insufficient predictor data were recorded.
Three youth aged <10 years with type 2 diabetes were excluded due to too much missing data and insufficient data (e.g., labs, medication use) to confirm their diabetes type.
Upon arrival, all data were screened in detail by a dedicated intensive care specialist for any missing information, insufficient detail, or any other queries.
†Some missing data owing to insufficient blood collection or laboratory technical problems.
Reasons for missing data stemmed from insufficient supplies or specimen volumes and were thus assumed to be missing at random [ 20]; with the exception of weight, which could be related to severity of illness and short-term mortality.
Without ruling out the effect of genetic differences between populations, this result suggests that greater amplification of fewer target sites was not a useful strategy to limit the amount of missing data resulting from insufficient read depth.
However, recognizing the limitations of NGS technology, Sanger sequencing to 'fill in' missing data from regions with insufficient coverage (e.g. regions where each bp is sequenced <10 20 times) is recommended.
VK tried to contact trial authors for clarification or insufficient of missing data when necessary and summarised data reported in multiple publications as one single data set.
Six analyses provided reasons for missing data, and there was often insufficient detail to be able to rule out selective reporting.
