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The search criteria were as follows: full tryptic specificity was required; two missed cleavages were allowed; carbamidomethylation was set as fixed modification; oxidation (M) was set as a variable modification; precursor ion mass tolerance was 10 ppm for all MS acquired in the Orbitrap mass analyzer; and fragment ion mass tolerance was 0.8 Da for all MS2 spectra acquired in the LTQ.
Trypsin was designated as the protease and up to two missed cleavages were allowed.
Trypsin was specified as the proteolytic enzyme and up to two missed cleavages were allowed.
Oxidized methionine, deamidation, carbamidomethyl cysteine, and phosphorylated serine, threonine and tyrosine were selected as variable modifications, and as many as 3 missed cleavages were allowed.
The following search criteria were employed: full tryptic specificity was required; two missed cleavages were allowed; Carbamidomethylation was set as fixed modification, whereas Oxidation (M), Phospho (ST), and Phospho (Y) were considered as variable modifications.
Two missed cleavages were allowed.
Two missed cleavages were permitted.
Up to two missed cleavages were allowed.
A maximum of two missed cleavages were allowed.
The maximum missed cleavages were set as 1.
Enzyme cleavage was set to trypsin, and maximally two missed cleavages were allowed.
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